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SCSR Fecal Cell Isolation System
Without
the need for invasive biopsies, researchers and clinicians can investigate
disease-related genetic markers and cell-surface markers and can monitor their
patients' nutritional or disease status. The SCSR Fecal Cell
Isolation system typically yields several million
cells per gram of stool sample, enabling downstream analyses including cell
culture, nucleic acid analysis, cell-surface analysis, and biopsy-free
cell-based archiving of a patient's natural history. Developed
over the past
20 years, this technology has been used to isolate cells from human,
rat, mouse, cattle, dog, cat, monkey, goat, and sheep samples.
DNA Extraction: Comparison
of extraction from 200mg whole stool and extraction from 200mg equivalent of
cells.
Compares yield after hold times of 0 hrs (direct extraction only), 3 hrs, and
24 hrs at room temperature.
Fecal Cell Isolates Left: Phase
Contrast
Right: Rhodamine-labeled Phalloidin (Actin Stain)
Cultured Colonocyte Left: DIC Right:
FITC-Labeled Anti-Human IgA
Fecal Cell Isolates: Left: Phase Contrast /Rhodamine-Phalloidin
Right: DIC/DAPI (DNA)
Fecal Cell Isolates Left: DIC Middle:
Rhodamine-Phalloidin Right: DAPI
PRODUCTS
SCSR™
is the only available kit for isolating cells from fecal samples. Our
preservative medium allows samples to be shipped at room temperature for up
to 5 days. The isolation procedure is a simple step-gradient centrifugation
followed by washing in PBS. Cells can be analyzed for protein, DNA, and
RNA and can be archived for future use. Tests show that DNA extraction
from stool is enormously improved with pre-isolation of cells. Our centrifugal
approach surpasses bead or column capture, because all cell types are isolated,
and no release step is required. [Click
for product page] [Click
for technical information]
We also offer SCSR-T™,
a general purpose preservative and room-temperature transport medium for biological
samples. It has been formulated to maintain the integrity and viability
of eukaryotic cells for downstream analysis. Samples preserved using SCSR-T
can be transported at room-temperature and used for cell isolation, cell culture,
DNA and RNA extraction, intracellular microbial analysis, analysis of viral
integration into the host genome, pharmacogenomics, nutrient-gene interaction
studies. SCSR-T™ is non-toxic and non-hazardous.
[Click
for product page] [Click
for technical information]
R&D
Our lab is located on the I-95 corridor between Baltimore and Washington, is
equipped for performing flow cytometry, cell isolation, nucleic acid extraction,
PCR, real-time PCR, tissue-culture. [Contact
us]
Adult
progenitor cells isolated from stool samples
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LLC. All rights reserved
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