|SCSR Cell Isolation - Photographs|
|Stomacher Bag Filtration|
Pour Sample into Strainer
Extract using Pipette
Place filter over 50-ml centrifuge tube
To avoid clogging, repeatedly aspirate and dispense sample using pipette
Slowly underlay cushion medium from the bottom of tube. Try to avoid bubbles and
disturbance of the interface.
|Appearance after Centrifugation|
The tube after centrifugation
Use pipette to remove supernatant, leaving 5-10 ml above interface
|Extracting the Interface|
Extract interface using plastic transfer pipette and dispense into a new tube. Slowly follow a downward spiral path along tube wall while extracting.
If there is a 'tinge' to the top of the cushion, extract a small amount of cushion as well.
Leave most of cushion with the pellet fraction. Fractions will be washed in the next step.
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